Events   About   Projects   Experiments   Texts   Blogs   Resources   Contact horizontal divider horizontal divider horizontal divider
katE sees red with Red Fluorescent Protein (RFP) Laura Cinti divider 12 Sep 12, 08:48 divider Write Comment divider

Single colony growth katE promoter fused to an RFP construct, day 14 using swarming plates.

One of the reasons for changing the oxidative stress construct, katE, to express red fluorescence, was to align the signification of stress with cultural understanding of colours. For instance, when humans are under stress, blood flushes to the face giving it a reddish colour particularly amongst those with paler skin. Also, red can be seen as an alerting colour and has been used as ‘official’ semantics in many apparatuses such as traffic lights, where it is used as opposed to green. While these factors play little biochemical role, they may impact our understandings. A more visual reason for using Red Fluorescent Protein (RFP) is its light pink colour visible in daylight.

katE red - bacteria blushes during stress from c-lab.

Similarly to the Green Fluorescent Protein (GFP) stress-reporting construct, the RFP construct (BBa_J06702) was assembled with katE promoter using idempotent design.
Cleaning oil spills with magnets Laura Cinti divider 12 Sep 12, 23:35 divider Comments (2) divider
© 2013 c-lab. All Rights Reserved. Sitemap | T&Cs | t: +31 (0)20 754 7207 e: Design and Implementation by c-lab (2013)