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  Going solid: From Fungi to Mushroom

Media containing wheat and PDY

As part of the bio-remediation artwork Transient Images that generates images while decolourising dye and then use a fungi (Pleurotis) to remediate the toxic by-product. Here, I explore the final step - the daunting task of producing an edible mushroom (oyster) from the fungi. 

Inoculation step

The main issue in going from fungi to mushroom is the time it takes to form a fruiting body. The papers we looked at suggested one month!

Three plugs

To make it happen, our idea was to make a semi-solid media containing Potato Dextrose Yeast (PDY) and wheat flakes. From a white looking petri dish, fungi grown on PDA, I would punch out three plugs and placed these in the flask with semi-solid media.

Sealed flask

The flask was sealed with foil and placed at 30°C. Is one month too long?

 
http://www.flickr.com/photos/c-lab/sets/72157629655857857/
 
Other References: C-LAB:blog: Britt Wray of Fluxmedia visits C-LAB
C-LAB:blog: Cells alive n' counting
C-LAB:blog: Cellulose from bacteria
C-LAB:blog: Going solid: From Fungi to Mushroom
C-LAB:blog: Growing Magnetospirillium Gryphiswaldense
C-LAB:blog: It's here - the Spring 2012 iGEM library
C-LAB:blog: KatE in a Jar
C-LAB:blog: katE sees red with Red Fluorescent Protein (RFP)
C-LAB:blog: Red Fluorescent Protein
C-LAB:blog: Sludge Bacteria - one drop
C-LAB:blog: Stress light in growth & swarming behaviour
C-LAB:event: ART FROM SYNTHETIC BIOLOGY
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C-LAB:event: THE TWO CULTURES: VISUAL ART AND THE SCIENCES C.1800-2011
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C-LAB:experiment: Bacteria Compass
C-LAB:project: Bacterial Light Sensor
C-LAB:project: Bacterial World
C-LAB:project: katE - Visualising Stress
C-LAB:project: KatEred
C-LAB:project: Stress-o-stat
C-LAB:project: Sugar Rush
C-LAB:project: Transient Images
 
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