Events   About   Projects   Experiments   Texts   Blogs   Resources   Contact horizontal divider horizontal divider horizontal divider
  Growing Magnetospirillium Gryphiswaldense

Preparing media for growing magnetospirillium gryphiswalden has been a real pain in the neck. Soil or pond based bacteria are fastidious organisms that are fussy. I had to go back to basics and try to make up the media of the stock provider. The pink colour is produced when mixing the oxidation-reduction indicator "resazurin" with potasium phosphate salt.


And this is just to make up the base...making vitamin and trace element solution was more involved.

After mixing, adjusting pH, sterilising and combining the various solution (base, vitamin, trace elements, electron donor and carbon source) parts we finally end up with a light pink/violet media. By adding nitrogen gas to a sealed vial, the media was ready to be inoculated. But will they grow??! 

 
http://www.flickr.com/photos/c-lab/sets/72157629212221010/
 
Other References: C-LAB:blog: Britt Wray of Fluxmedia visits C-LAB
C-LAB:blog: Cells alive n' counting
C-LAB:blog: Cellulose from bacteria
C-LAB:blog: Going solid: From Fungi to Mushroom
C-LAB:blog: Growing Magnetospirillium Gryphiswaldense
C-LAB:blog: It's here - the Spring 2012 iGEM library
C-LAB:blog: KatE in a Jar
C-LAB:blog: katE sees red with Red Fluorescent Protein (RFP)
C-LAB:blog: Red Fluorescent Protein
C-LAB:blog: Sludge Bacteria - one drop
C-LAB:blog: Stress light in growth & swarming behaviour
C-LAB:event: ART FROM SYNTHETIC BIOLOGY
C-LAB:event: ART-SCIENCE TALK
C-LAB:event: BIOART FORUM
C-LAB:event: CAGE RATTLING #1: KILL SWITCH
C-LAB:event: DO IT TOGETHER BIO #5: SYNTHETIC BIOLOGY ART
C-LAB:event: GRADUATE SCHOOL LAUNCH
C-LAB:event: INTO THE LABS
C-LAB:event: RE-NEW DIGITAL ARTS FESTIVAL
C-LAB:event: SYNTHETIC BIOLOGY SOCIETY KICK OFF EVENT
C-LAB:event: THE TWO CULTURES: VISUAL ART AND THE SCIENCES C.1800-2011
C-LAB:event: YOUNG SYNTHETIC BIOLOGISTS - YSB 1.0
C-LAB:experiment: Bacteria Compass
C-LAB:project: Bacterial Light Sensor
C-LAB:project: Bacterial World
C-LAB:project: katE - Visualising Stress
C-LAB:project: KatEred
C-LAB:project: Stress-o-stat
C-LAB:project: Sugar Rush
C-LAB:project: Transient Images
 
Post Comment:
 
Name (required)  
Email (required - not displayed)  
Website

Letter 5 and 10 in the word above (required)  
To:
 
From:
 
© 2013 c-lab. All Rights Reserved. Sitemap | T&Cs | t: +31 (0)20 754 7207 e: info@c-lab.co.uk Design and Implementation by c-lab (2013)