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  Red Fluorescent Protein

I decided to move my stress promoter in front of an mCherry or red flourescent gene, and make a genetic construct that could also produce what looks like a red "ring of fire" (above). I am hopeful that what is observed at the bottom of this flask contains cells expressing red fluorescent proteins (RFP) under control of my genetic stress switch.

After the cloning, cells where grown overnight and centrifuged down to make a dense concentrate. I will verify that the construct is correct, but the strong red colour is at least a hopeful indicator.

Last year, I made a green fluorescent construct but RFP can be more difficult to express for bacteria. Despite this, once expressed, I think RFP is a more dramatic identifier.

 
http://www.flickr.com/photos/c-lab/sets/72157629576822661/
 
Other References: C-LAB:blog: Britt Wray of Fluxmedia visits C-LAB
C-LAB:blog: Cells alive n' counting
C-LAB:blog: Cellulose from bacteria
C-LAB:blog: Going solid: From Fungi to Mushroom
C-LAB:blog: Growing Magnetospirillium Gryphiswaldense
C-LAB:blog: It's here - the Spring 2012 iGEM library
C-LAB:blog: KatE in a Jar
C-LAB:blog: katE sees red with Red Fluorescent Protein (RFP)
C-LAB:blog: Red Fluorescent Protein
C-LAB:blog: Sludge Bacteria - one drop
C-LAB:blog: Stress light in growth & swarming behaviour
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C-LAB:experiment: Bacteria Compass
C-LAB:project: Bacterial Light Sensor
C-LAB:project: Bacterial World
C-LAB:project: katE - Visualising Stress
C-LAB:project: KatEred
C-LAB:project: Stress-o-stat
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