In the last week, I have been reflecting and re-exploring growth and swarming in E.coli using time-lapse and a synthetic biology construct (katE) I created last year that produces fluorescence (GFP) in response to oxidative stress. Unfortunately, the time-lapse crashed due to an electrical outrage, however, the growth did produce a rather stunning mega colony (below). Leaving it in an incubator for an additional 2 days a swarming behaviour began to emerge (above). I hope to catch this using time-lapse soon.
